Sunday, January 30, 2011

BACTERIOPHAGE IN VITRO PACKAGING

(1977) Packaging recombinant DNA molecules into.

In Vitro Packaging of DNA Basic DNA and RNA Protocols, Volume 58: 22 Dale. Recombinant molecules constructed using cosmids are incorporated into bacteriophage using in vitro packaging extracts and introduced with high efficiency. In vitro packaging of bacteriophage T4 DNA. doi:10.1126/science.3107124. A small RNA of Bacillus subtilis bacteriophage phi 29 is shown to have a novel and essential role in viral DNA packaging in vitro. In vitro packaging into phage T4 particles and specific. The viruses themselves can be cut and inserted into. Bacteriophage lambda can accept large inserts of foreign DNA and infect E. "A small viral RNA is required for in vitro packaging of bacteriophage phi 29 DNA".

No comments:

Post a Comment